Liu Ya-ci; Zhang Zhao-ji; Zhao Xin-yi; Wen Meng-tuo; Cao Sheng-wei; Li Ya-song

doi:10.19637/j.cnki.2305-7068.2021.04.004

doi: 10.19637/j.cnki.2305-7068.2021.04.004

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出版历程
- 收稿日期: 2021-07-09
- 录用日期: 2021-10-21
- 网络出版日期: 2021-10-29
- 刊出日期: 2021-12-15

Arsenic contamination caused by roxarsone transformation with spatiotemporal variation of microbial community structure in a column experiment

Ya-ci Liu^{1, 2},
Zhao-ji Zhang^{1, 2},
Xin-yi Zhao³,
Meng-tuo Wen⁴,
Sheng-wei Cao^{1, 2},
Ya-song Li^{1, 2
, ,}

1.
Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences, Shijiazhuang 050061, China
2.
Key Laboratory of Groundwater Remediation of Hebei Province and China Geological Survey, Shijiazhuang 050061, China
3.
Hebei Hydrology Engineering Geological Survey Institute Co. LTD, Shijiazhuang 050061, China
4.
China University of Geosciences, Beijing 100083, China

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Corresponding author: liyasong712@126.com

摘要

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Abstract: Arsenic contamination from roxarsone in livestock manure is common, and livestock manure continuously accumulates in the open environment. Evaluations of the environmental processes of As mobilization and transformation are critical for predicting the fate of As compounds after roxarsone degradation. In this study, spatiotemporal variations in As species and microbial community structure were characterized using laboratory column experiments with background soil collected from Yanggu County (northern Shandong Plain, China), a region of intense poultry production. Organic and inorganic arsenic were detected by high-performance liquid chromatography (HPLC) and HPLC with hydride generation atomic fluorescence spectrometry (HPLC-HG-AFS), respectively. High-throughput sequencing technology was used to describe microbial diversity. Results showed that roxarsone was transformed completely within 7 days, and As(Ⅲ) and As(Ⅴ) were the major degradation products. The concentration of As(Ⅲ) was much lower than that of As(Ⅴ). The As(Ⅲ) concentration increased significantly after Day 14, whereas the As(Ⅴ) concentration increased significantly after Day 84, indicating that As(Ⅲ) was initially produced. The microbial community structure changed significantly as roxarsone transformed into various As compounds. A critical and dominant bacterial strain, norank_f__Family_XVⅢ, was found to be related to the degradation of roxarsone into As(Ⅲ). This study improves our understanding of the fate of As species released from poultry litter to soil and groundwater, which is a threat to human health and environment.
- Arsenic /
- Roxarsone /
- Spatiotemporal variation /
- Microbial community

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Figure 1. The particle size distribution of soil

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Figure 2. Design of the one-dimensional soil column

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Figure 3. Spatiotemporal variation in As(Ⅲ) and As(Ⅴ) concentrations

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Figure 4. The variation in alpha diversity indices over time. CV: coefficient of variation.

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Figure 5. Principal coordinate analysis (PCoA) of soil samples on genus level. Samples were named by sampling time (Day 7, 14, 28, 56, 84, or 112) and depth (d-1, d-2, d-3, d-4, d-5, d-6, and d-7 representing 5 cm, 15 cm, 25 cm, 35 cm, 45 cm, 55 cm, and 65 cm, respectively).

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Figure 6. Relative microbial community abundance on genus level of different soil depths at six sampling times

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Figure 7. The relative abundance of strain norank_f__Family_XVⅢ over time

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Figure 8. Spearman correlation heatmap between dominant species and environmental factors. Red represents a positive correlation and blue represents a negative correlation; *: 0.01<P ≤0.05, **: 0.001<P ≤0.01, ***: P ≤0.001

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Figure 9. Phylogenetic tree of the dominant strains on genus level (The bar chart on the right shows the abundance of strains at different sampling times.)

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Table 1. The chemical composition of soil

Chemical compounds	SiO₂	Al₂O₃	CaO	Fe₂O₃	MgO	K₂O	Na₂O	TiO₂	Others
Proportion (%)	64.65	15.22	6.54	4.46	2.89	2.68	1.81	0.802	0.948

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Table 2. Correlation analysis of arsenic (As) species and chemical elements

	As(Ⅴ)		As(Ⅲ)		Nitrogen content		TOC
	Pearson correlation	Significance (bilateral)	Pearson correlation	Significance (bilateral)	Pearson correlation	Significance (bilateral)	Pearson correlation	Significance (bilateral)
Mg	−0.596**⁽¹⁾	0.000	−0.333*⁽²⁾	0.031	−0.041	0.798	−0.180	0.255
Al	−0.430**	0.005	−0.232	0.139	−0.163	0.303	−0.144	0.361
P	−0.029	0.853	0.011	0.947	−0.111	0.482	0.141	0.375
K	−0.220	0.161	−0.101	0.526	−0.227	0.149	−0.125	0.429
Ca	−0.351*	0.023	−0.181	0.252	−0.370*	0.016	−0.275	0.078
Mn	−0.282	0.070	−0.089	0.576	−0.290	0.063	−0.224	0.154
Fe	−0.148	0.349	−0.024	0.881	−0.271	0.082	−0.066	0.676
Cu	0.013	0.934	0.090	0.571	−0.296	0.057	0.014	0.927
Zn	−0.196	0.213	−0.071	0.656	−0.300	0.053	−0.214	0.174
Pb	0.191	0.225	0.209	0.184	−0.246	0.117	0.105	0.509
As(Ⅴ)	1.000		0.448**	0.003	−0.079	0.618	0.303	0.051
As(Ⅲ)	0.448**	0.003	1.000		−0.182	0.249	0.100	0.529
Nitrogen content	−0.079	0.618	−0.182	0.249	1.000		0.443**	0.003
TOC	0.303	0.051	0.100	0.529	0.443**	0.003	1.000
Note: (1) ** Correlation is significant at the 0.01 level; (2)* Correlation is significant at the 0.05 level.

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