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Transformation of ammonium nitrogen and response characteristics of nitrifying functional genes in tannery sludge contaminated soil

Kong Xiang-ke Zhang Zi-xuan Wang Ping Wang Yan-yan Zhang Zhao-ji Han Zhan-tao Ma Li-sha

Kong XK, Zhang ZX, Wang P, et al. 2022. Transformation of ammonium nitrogen and response characteristics of nitrifying functional genes in tannery sludge contaminated soil. Journal of Groundwater Science and Engineering, 10(3): 223-232 doi:  10.19637/j.cnki.2305-7068.2022.03.002
Citation: Kong XK, Zhang ZX, Wang P, et al. 2022. Transformation of ammonium nitrogen and response characteristics of nitrifying functional genes in tannery sludge contaminated soil. Journal of Groundwater Science and Engineering, 10(3): 223-232 doi:  10.19637/j.cnki.2305-7068.2022.03.002

doi: 10.19637/j.cnki.2305-7068.2022.03.002

Transformation of ammonium nitrogen and response characteristics of nitrifying functional genes in tannery sludge contaminated soil

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  • Figure  1.  Transformation of ammonium nitrogen in contaminated silt soil and fine sand under different pollution loads

    (A:FW1;B:SW1;C:FW2;D:SW2;E:FW3;F:SW3)

    Figure  2.  Richness characteristics of amoA and nxrA genes in silt soil under different pollution loads

    (A: amoA;B: nxrA; different lowercases indicate significant differences between the data (P < 0.05))

    Figure  3.  Richness characteristics of amoA and nxrA genes in silt soil and fine sand under low pollution load

    (A: amoA;B: nxrA; different lowercases indicate significant differences between the data (P < 0.05))

    Figure  4.  Transformation of ammonia nitrogen in contaminated silt soil under anaerobic condition

    (A: OFW1; B: OFW2; C: OFW3)

    Figure  5.  Genes richness characteristics of amoA and nxrA under different redox condition

    (A: amoA;B: nxrA; different lowercases indicate significant differences between the data (P < 0.05))

    Figure  6.  S-growth fitting curve of nitrate accumulation process under different environmental condition

    (A: pollution load; B: soil texture; C: redox condition)

    Table  1.   Basic physical and chemical properties of the tannery sludge and soil

    TypeTOC(%)pHCEC(mol/kg)Fe2O3(%)Cr(III)(mg/kg)NH4+-N(mg/kg)NO3-N(mg/kg)
    Silt soil0.177.6113.66.133520<5
    Fine Sand0.097.259.753.481712<5
    Tannery Sludge14.37.67--3080016500500
    下载: 导出CSV

    Table  2.   Culture conditions of tannery sludge contaminated soils

    TypeSampleCulture conditionSampleCulture condition
    Tannery contaminated-Silt soilFW1Aerobic, Sludge addition 15%OFW1Anaerobic, Sludge addition 15%
    FW2Aerobic, Sludge addition 30%OFW2Anaerobic, Sludge addition 30%
    FW3Aerobic, Sludge addition 50%OFW3Anaerobic, Sludge addition 50%
    Tannery contaminated-Fine SandSW1Aerobic, Sludge addition 15%
    SW2Aerobic, Sludge addition 30%
    SW3Aerobic, Sludge addition 50%
    下载: 导出CSV

    Table  3.   Quantitative PCR primer and reaction procedure

    Objective GenesSegment SiteSarterSequence(5’-3’)Reaction procedure / Condition
    amoA491AmoA-1FGGGGTTTCTACTGGTGGT95°C for 3 min × 1cycle
    AmoA-2RCCCCTCKGSAAAGCCTTCTTC95°C for 30 s,56°C for 30 s,72°C for 40 s×35cycles
    nxrA324F1norACAGACCGACGTGTGCGAAAG95°C for 3 min × 1cycle
    R1norATCYACAAGGAACGGAAGGTC95°C for 30 s,56°C for 30 s,72°C for 40 s×35cycles
    下载: 导出CSV

    Table  4.   Sigmodial fitting parameters of nitrate accumulation process under different environment conditions

    Reaction ConditionA1A2t0
    (d)
    A2-A1
    (mg/kg)
    (A2-A1)/(4dt)
    (mg/kg·d)
    R2
    FW10.45180.0937.79179.6412.720.988
    FW211.01387.4239.23376.4132.560.999
    FW31.21761.8951.71760.6834.390.997
    SW21.39307.4347.83306.0415.610.966
    OFW20.54145.6849.71145.145.870.999
    下载: 导出CSV
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出版历程
  • 收稿日期:  2021-12-16
  • 录用日期:  2022-06-06
  • 刊出日期:  2022-09-15

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