Abstract: This paper aims to study the influence of the number and distribution of drill samples on the simulation accuracy of the lithology field. This research mainly applies the variation function method in geo-statistics, and determines important indicators such as the variation, and then the lithology field is simulated by sequence index simulation. It is shown that (1) simulation error decreases with the increase of sampling density; (2) at the scale and complexity of this study, when the sampling density reaches 40 /km2, the average error of the lithology field simulation can be less than 2.0%; (3) in the study mode of examples, the simulation results of random sampling in the whole region are the most ideal, with an average error of 5.4%. The average error of the simulation results of the centralized sampling is about 10 times that of the random sampling method; (4) known from the influence analysis of the degree of study sample unevenness influence on the imitation results, under the same sample size, the simulation error decreases with the increase of the most adjacent index. When the nearest index reaches 1, the simulation error will be less than 6%, and the error variable range is within 3%.